Detection of enterochromaffin cells by the luminescence method with lighting from the side
Khibin, L.S.
Arkhiv Anatomii Gistologii i Embriologii 73(11): 113-115
1977
ISSN/ISBN: 0004-1947 PMID: 597046 Document Number: 117409
A method is suggested to detect luminescence of enterochromaffin cells of the intestinal tract after a routine fixation of sections in 10% formalin, imbedding in paraffin, and examination under the microscope when the light is directed to the preparation laterally, at the angle of 30--40 degrees. The sections fixed on the glass are deparaffined (xyline, chloroform), dried quickly in the air until "bleaching" and mounted in vaseline oil. Under a light microscope, with lateral illumination of the preparation, enterochromaffin cells have golden yellow luminescence. This simple method makes it possible to study enterochromaffin cells in stained preparations and can be useful for cytofluorometric studies.