The use of dodecyl-di (beta-oxyethyl) benzylammonium chloride (Bactofen) as a decontaminant in the cultural examination for detection of the Koch bacillus in sputum
Carpi Torelli, P.; Tortoli, E.
Quaderni Sclavo di Diagnostica Clinica E di Laboratorio 16(2): 196-205
1980
ISSN/ISBN: 0033-4979 PMID: 6787655 Document Number: 160325
We tried to see whether it was possible to use Bactofen to decontaminate sputum for culture of Mycobacterium tuberculosis. Bactofen showed very little bactericidal action on tubercle bacilli, lower than Desogen, and selectively destroyed many contaminants, more actively than benzalkonium chloride. Digested sputum decontaminated with 0.2% Bactofen and then applied to culture media without centrifugation gave good results in growth of colonies.