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Double-stranded DNA replication intermediates in human malignant melanoma cells

Lönn, U.; Lönn, S.

Cancer Research 40(10): 3792-3797

1980

ISSN/ISBN: 0008-5472
PMID: 7438062
Document Number: 158795
DNA synthesis was investigated in human malignant melanoma cells. The DNA was extracted in a neutral nondenaturing buffer at 25 degrees and characterized by agarose gel electrophoresis. Radioactive DNA pulse labeled for 10 to 30 min was released from the chromosomes in the form of double-stranded fragments. The release of the fragments from the chromosomes is prevented by lysing the cells at 0 degrees instead of at 25 degrees suggesting that the release is enzymatic. The size of the fragments ranges between 4.6 and 5.6 x 10(6) daltons. After a time lag, the fragments are joined together to produce a high-molecular-weight DNA.

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