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Interconvertible hairpin structure found in the replication origin of phage G4 single-stranded DNA

Hirao, I.; Nishimura, Y.; Ishida, M.; Watanabe, K.; Miura, K.

Nucleic Acids Symposium Series 21: 13-14

1989

ISSN/ISBN: 0261-3166
PMID: 2608461
Document Number: 340464
We found a synthetic GCGAAAGC fragment with a mobility greater than that of other oligodeoxyribonucleotides in gel electrophoresis to take on a stable hairpin structure possessing two terminal G-C base pairs. The GCGAAAGC sequence is also found in the replication origin of phage G4 single-stranded DNA, but the hairpin structure originally proposed differs from that of the GCGAAAGC fragment we have studied. Possibility of rearrangement of the secondary structure in the replication origin of phage G4 was examined in relation to its replication initiation mechanism.

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