High-performance liquid chromatographic separation of natural adenosyl-sulphur compounds
Zappia, V.; Galletti, P.; Porcelli, M.; Manna, C.; Ragione, F.D.
Journal of Chromatography 189(3): 399-405
1980
ISSN/ISBN: 0021-9673 PMID: 7380939 Document Number: 158581
A rapid, sensitive and specific high-performance liquid chromatographic method for the simultaneous separation of natural adenosyl-sulfur compounds was developed. The compounds were separated by using the strong cation-exchange resin Partisil 10 SCX with isocratic elution. The adenosyl-compounds were monitored by a UV detector operating at 254 nm. Sensitivity was greater than 50 pmol for all compounds tested and standard curves were linear for concentrations of up to 50 nmol. The method can be applied to biological samples for the estimation of [Saccharomyces cerevisiae] S-adenosyl-L-methionine, S-adenosyl-L-homocysteine and S-adenosyl-(5')-3-methyl-thiopropylamine and for measurement of enzyme activities involving the above-mentioned compounds.