Development and optimisation of a high-performance liquid chromatographic assay for tioconazole and its potential impurities. I. Selection of separation conditions
Wright, A.G.; Fell, A.F.; Berridge, J.C.
Journal of Chromatography 464(1): 27-38
1989
ISSN/ISBN: 0021-9673 PMID: 2715249 Document Number: 331858
Statistical mixture design techniques have been utilized to develop an isocratic high-performance liquid chromatographic separation of 1-{2-[(2-chloro-3-thienyl)- methoxy]-2-(2,4-dichlorophenyl)ethyl}-1H-imidazole (tioconazole) and its potential impurities. By using suitable quality criteria such as reduced plate height, peak asymmetry and selectivity for the interrogation of chromatograms the variables producing best column efficiency and greatest selectivity have been identified. The optimum separation for these variables has been located by the sequential simplex approach and confirmed by response surface mapping.