Separation of blood cells from normal blood and blood of patients with various malignant blood diseases using separation media
Koubek, K.; Herzog, P.; Libánsky, J.
Folia Haematologica 107(1): 41-53
1980
ISSN/ISBN: 0323-4347 PMID: 6157623 Document Number: 158170
Differential counts of normal and leukemic cells separated from human peripheral blood were compared, using 3 separation media: verografin, verografin-ficoll and isopaque-ficoll (lymphoprep), density 1.077 g/ml. In the separation, the solution of verografin (SPOFA) or verografin-ficoll yields analogous cell counts to isopaque-ficoll solution. The separation of leukemic cells is similar on all 3 separation media with considerable packing of some cells of the myeloid line. The blood cells separated from peripheral blood of normal donors on 3 various separation media correlate also in T and rosette tests.