Effect of citrate, tartrate, succinate and phosphate ions on the penetration of phosphorus-labelled anions into erythrocytes
Gomulkievich, I.; Popdimitrova, N.; Kunchev, N.; Zlatarev, G.
Eksperimentalna Meditsina i Morfologiia 19(2): 92-97
1980
ISSN/ISBN: 0367-0643 PMID: 7379734 Document Number: 157755
Membrane permeability for different ions may successfully be evaluated under conditions of ionic equilibrium, which provides osmotic equilibrium in the cell and steadiness of its volume. The present investigation was promoted on red cells of experimental animals under conditions of ionic equilibrium, using P32 with up to 30 microCi activity. Unidirectional penetration of ions in the red cells was studied. The speed constant K0 was derived from the graphic relation InA=f(t) as an angular coefficient at the starting moment. The type of ions was shown to influence K0 value, which was maximal in the presence of tartarate ions in the suspension medium and minimal in the presence of succinate ions. This study demonstrated that the activation energy does not depend on the ions in the solution. The results are highly suggestive of the existence of differences in the mechanism of penetration of different ions through the red cell membrane.