Isoenzymes of succinate dehydrogenase in measles virus-infected monkey kidney cells
Prahoveanu, E.; Eşanu, V.; Sorodoc, Y.
Virologie 31(1): 41-45
1980
ISSN/ISBN: 0253-181X PMID: 6769238 Document Number: 157364
Succinate dehydrogenase (SDH) isoenzyme patterns were analyzed in R6CA monkey kidney cells inoculated with wild and attenuated measles virus. Cell homogenates were prepared either in a sucrose--Triton X-100 mixture, or in sucrose alone. In both cases the most important SDH isoenzyme pattern alterations were observed in the homogenates of cells infected with wild measles virus at 72 hours post inoculation. Similar alterations occurred in homogenates of cells infected with the attenuated strain after a delay of about 100 hours. In all the experimental variants the use of Triton X-100 allowed the visualization of a larger number of isoenzyme bands.