Preparation of cellulolytic enzymes from Geotrichum candidum
Tiunova, N.A.; Rodionova, N.A.; Martinovich, L.I.; Gogolev, M.N.
Prikladnaia Biokhimiia i Mikrobiologiia 16(2): 185-190
1980
ISSN/ISBN: 0555-1099 PMID: 6770360 Document Number: 157142
The ability of different fungi cultivated on cellulose to synthesize extracellular cellulase was examined. The fungus Geotrichum candidum strain 3c was the most active among the fungi tested, including Trichoderma lignorum. The formation of cellulolytic enzymes in the culture filtrate of G. candidum occurred, when the entire glucose and the major portion of reducing substances were utilized. The formation of cellulases depended on the content of ammonium ions. The enzyme accumulation reached the highest level at the stationary phase of the culture growth and depended on the inoculum amount. When the inoculum was used as a submerged culture at a concentration of 3%, accumulation of C1-enzyme endo-(1 .fwdarw. 4).beta.-glucanases, and .beta.-glucosidases reached maximum by 44, 52 and 56 h of cultivation. The fats that are frequently used as a foam catcher inhibited the cellulase formation by the above strain. From the filtrate cellulolytic enzymes were isolated via spray drying and precipitation with 3 ethanol volumes to yield 6.5 and 5.0 g/l, respectively. The cellulases when stored at 5.degree. C did not lose their activity for a year and became inactivated by 40-50% in 5 yr.