Use of unscheduled DNA synthesis in freshly isolated human intestinal mucosal cells for carcinogen detection
Freeman, H.J.; San, R.H.
Cancer Research 40(9): 3155-3157
1980
ISSN/ISBN: 0008-5472 PMID: 7427933 Document Number: 156498
Mucosal cells freshly isolated from human intestine with pronase retain the capacity to undergo DNA repair synthesis (unscheduled DNA synthesis) during a 2-hr exposure to the carcinogen, N-methyl-N'-nitro-N-nitrosoguanidine, and the procarcinogen, aflatoxin B1. This procedure combining the use of human intestinal mucosal cells and the measurement of unscheduled DNA synthesis may provide a highly relevant and convenient test system for the detection of cell-specific, direct-acting, and activation-dependent chemical carcinogens. The use of whole-cell preparations in such in vitro studies may be of additional significance in view of growing evidence for artefactual metabolism by subcellular fractions.