The urinary cotinine and serum 25 hydroxyvitamin D levels in male smokers
Buacharoen, T.; Kongtip, P.; Tharpoophasiam, P.; Chantanakul, S.; Sujirarat, D.
Journal of the Medical Association of Thailand 95(Suppl 6): S87-S93
2012
ISSN/ISBN: 0125-2208 PMID: 23130493 Document Number: 15640
To assess urinary cotinine and the effects of smoking on 25(OH)D levels in 67 male smokers. Urine and blood specimens were analyzed for cotinine and serum 25 hydroxyvitamin D (25(OH)D) concentrations by high performance liquid chromatography (HPLC) and chemiluminescent immunoassay, respectively. The accuracy, precision and detection limit of the HPLC method were also tested. The detection limit of urinary cotinine was 0.02 microg/ml. The recoveries of cotinine concentrations of 0.15-2.0 microg/ml were greater than 95%. Only 23.9% of smokers had sufficient levels of serum 25(OH)D at least 30 ng/ml. The smokers were divided into dairy co-operative smokers and other smokers. The average urinary cotinine concentrations of 1,421.42 and 1,866.52 microg/g creatinine were not significant different in dairy co-operative smokers and other smokers whereas the average 25(OH)D of 29.09 and 22.65 ng/ml, respectively, were significantly different at p-value of < 0.001. The 42.86% and 10.26% of the diary co-operative smokers and other smokers had sufficient serum 25(OH)D levels to prevent osteoporosis.