Murine lymphocyte alloantigens. I. The Ly-6 locus
Potter, T.A.; McKenzie, I.F.; Morgan, G.M.; Cherry, M.
Journal of Immunology 125(2): 541-545
1980
ISSN/ISBN: 0022-1767 PMID: 6156206 Document Number: 156386
The murine Ly-7 locus was identified by the reactivity of alloantisera prepared as B6.C-H-2d X CXBG)F1 anti-CXBK. Most strains, except C57BL/6 and related strains, were Ly-7.2+. Absorption analysis demonstrated that the Ly-7 specificity was only found on lymphocytes. Direct testing by cytotoxicity and protein-A rosetting showed that Ly-7 is expressed in very low amounts on thymocytes, but is readily detectable on more mature T and B cells (including antibody-forming cells), with the greatest expression on B cells. Typing backcross mice with Ly-7.2, Ly-4.2 and H-2.33 antisera showed that Ly-7 is not linked to the Ly-4 locus or H-2 complex. In raising the antisera by hyperimmunisation, it was found that the antibody response to the Ly-7.2 specificity was influenced by an H-2-linked immune-response gene.