Ability of vancomycin-group antibiotics to induce or to inhibit thrombocyte agglutination
Rák, K.; Boda, Z.; Sztaricskai, F.
Antibiotiki 25(8): 595-606
1980
ISSN/ISBN: 0003-5637 PMID: 6250464 Document Number: 155949
The effect of 4 vancomycin antibiotics on factor VIII-dependent agglutination of thrombocytes was studied. Significant similarity, quantitative and qualitative, between ristocetin and ristomycin was found. In this connection, ristomycin may be used for determination of the ristocetin cofactor. Actinoidin and vancomycin inhibited agglutination of platelets induced by ristocetin or ristomycin in platelet-enriched plasma with citrate or EDTA the same as in the system containing platelets treated with formalin and did not inhibit agglutination induced by bovine factor VIII. The 4 antibiotics induced precipitation of plasma protein. Vancomycin was most active and actinoidin was least active in this respect. Ristocetin and ristomycin also possessed such capacity, the effect of the latter being higher. Actinoidin and vancomycin did not prevent the immediate effect of light absorption increasing due to the addition of ristocetin or ristomycin to fixed platelets in concentrations completely inhibiting aggulutination of platelets in the presence of the protein cofactor. Inhibition of this direct effect of ristocetin and ristomycin was observed only at higher concentrations, which indicated that this effect was probably not associated with agglutination. Methylated carboxylic groups and free hydroxyls of phenol may play the main role in ristomycin binding with thrombocytic membrane and/or protein cofactor.