Vital microscopy of the lung
Kudriashov, G.M.; Smirnov, I.K.; Shekhunov, E.D.; Iakubenas, A.V.
Arkhiv Anatomii Gistologii i Embriologii 79(7): 93-97
1980
ISSN/ISBN: 0004-1947 PMID: 7416995 Document Number: 154275
In order to develop an optimal method for prevital microscopy of the lung, in 12 experiments performed in mongrel dogs advantages of three methods of contact microscopy were compared: dark-field, polarization and fluorescent-absorptive. The results on investigating the lung microstructure and microcirculation demonstrated usefulness of all the three methods. Nevertheless, in order to estimate the lung microstructure and microcirculation, the dark-field method is the most expedient, to reveal the pleural vascular network, the best results are obtained with the fluorescent-absorptive microscopy. It is necessary to fix the part examined under the objective by means of a special fixator-sticker that does not traumatize the lung parenchyma and does not disturb the respiratory microcirculation.