A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines
Kearney, J.F.; Radbruch, A.; Liesegang, B.; Rajewsky, K.
Journal of Immunology 123(4): 1548-1550
1979
ISSN/ISBN: 0022-1767 PMID: 113458 Document Number: 153327
We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
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