Characteristics of human-mouse hybrid cell lines and the changes induced in them by the poliomyelitis virus
Karmysheva, V.I.; Mustafina, A.N.; Koroleva, G.A.; Lashkevich, V.A.
Tsitologiia 28(7): 713-719
1986
ISSN/ISBN: 0041-3771 PMID: 3020749 Document Number: 271892
Cells of the four hybrid lines between continuous mouse cells Rag and human diploid embryonal fibroblasts were polymorphic and had mitotic activity in fully formed monolayers. Most of the these mitoses were pathological. Hybrid cells examined 8 months after hybridization were susceptible to the poliomyelitis virus infection with partial cytopathologic effect, they produced virus antigens and the infectious virus. Small hybrid cells displayed a more pronounced cytopathologic effect than did big, polynuclear and mitotic cells. Hybrid cells that were passaged 1.5 months after infection did not excrete any infectious poliovirus but contained poliovirus antigens.