Ultrastructural changes in the cells of a nonsynchronous Pseudomonas aeruginosa culture occurring under the influence of chlorhexidine bigluconate
Litovchenko, P.P.
Zhurnal Mikrobiologii Epidemiologii i Immunobiologii 8: 83-86
1979
ISSN/ISBN: 0372-9311 PMID: 115182 Document Number: 152819
Total preparations of P. aeruginosa (strain 65) were studied after contrastive treatment with 2% phosphato-tungstic acid and 2% uranyl acetate; ultrathin sections of bacteria treated with chlorhexidine bigluconate in various concentrations and fixied by the method of Hoffmann et al. were also studied with the use of an electron microscope IEM 100 v. Structural and morphological changes depending on the concentration and the time of action of the antiseptic were discovered; these changes were manifested by bacterial lysis and coagulation, the lossening of the cell wall with revealing its five-layer structure.