Use of immobilized proteinases for purification of sheep smallpox virus
Shedrin, E.L.; Prostiakov, A.P.; Tsygankova, S.I.; Skalinskiĭ, E.I.; Zinkevich, O.D.
Biokhimiia 44(10): 776-778
1979
ISSN/ISBN: 0320-9725 PMID: 228769 Document Number: 152291
A possible use of papain and trypsin immobilized on Biogel P-100 for purification of DNA-containing sheep smallpox viruses was studied. It was shown that the enzymes depolymerize tissue proteins, resulting in a 90% purification of the viruses without causing any considerable morphological changes in the virion structure. It was concluded that fixed proteinases can be effectively used at the final stage of purification of tissue virus-containing suspensions.