A small-volume thin-layer spectroelectrochemical cell for the study of biological components
Anderson, C.W.; Halsall, H.B.; Heineman, W.R.
Analytical Biochemistry 93(2): 366-372
1979
ISSN/ISBN: 0003-2697 PMID: 223467 Document Number: 152112
A small-volume (.apprx. 60 .mu.l) spectroelectrochemical cell was constructed and characterized. The working electrode is Au minigrid and the spectral window is from 170 nm-2 .mu.m. The solution is held in a thin layer around the working electrode, providing the advantages of thin-layer electrochemistry. The cell can be constructed from common materials in about 6 h. The cell also has flow-through and vacuum capabilities, with only minor modifications. The cell was characterized by cyclic voltammetry and coulometry on ferri-ferrocyanide and spectroelectrochemistry on cytochrome c and o-tolidine. The optical and electrochemical windows of this cell, as well as its convenient and versatile operation, make it useful for the study of a wide variety of biological redox components.