Induction of rat hepatic epoxide hydratase by dietary antioxidants
Kahl, R.; Wulff, U.
Toxicology and Applied Pharmacology 47(2): 217-227
1979
ISSN/ISBN: 0041-008X PMID: 452018 Document Number: 149458
Supplementation of rat diet with butylated hydroxytoluene (BHT), butylated hydroxyanisole or ethoxyquin resulted in increased liver epoxide hydratase activity. The increase was obvious at 0.1% and amounted to 200-400% at 0.5%. Increased activity was accompanied by increased proportion of the epoxide hydratase band in SDS pyrene to calf thymus DNA was less effectively catalyzed by liver microsomes from animals fed antioxidants. The depression of covalent binding was marked after combined treatment with antioxidants and 3-methylcholanthrene. The shift in the microsomal enzyme pattern caused by antioxidants may be related to their inhibitory effects on chemical carcinogenesis.