Study of the transmembrane potential of mitochondria using cyanine dyes

Pechatnikov, V.A.; Rizvanov, F.F.; Turchina, S.L.

Biofizika 24(1): 178-179

1979


ISSN/ISBN: 0006-3029
PMID: 219905
Document Number: 149195
Changes in fluorescent intensity of cyanine dyes dis-C3-(5) and dis-C2-(5) were measured in suspension of rat liver mitochondria upon the development of the energized state by adding succinate or ATP. It has been shown that these dyes possess a rotenone-like effect but they do not damage the inner mitochondrial membrane. The data on ATP synthesis and Ca2+ transport correspond to the basic aspects of the chemiosmotic conception.

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