Protein concentration in urine of normal dogs
Barsanti, J.A.; Finco, D.R.
American Journal of Veterinary Research 40(11): 1583-1588
1979
ISSN/ISBN: 0002-9645 PMID: 525877 Document Number: 148481
Protein concentration was measured by the Coomassie brilliant blue G-250 (CBB) dye-binding method and the trichloroacetic acid-ponceau S (TCA-PS) method in 193 urine samples from 157 dogs that had no evidence of disease of the urinary tract. Protein concentration in 145 random urine samples was similar with the CBB (mean 23 mg/dl, normal range 4-64 mg/dl) and TCA-PS (mean 20 mg/dl, normal range 4-95 mg/dl) methods. Significant difference in urine protein concentration was not found among samples collected by midstream voluntary voiding, cystocentesis or catheterization. Protein concentrations in urine from male and female dogs were not significantly different (P > 0.05) when samples were collected by catheterization or cystocentesis. A significantly greater (P < 0.01) protein concentration was found in urine voluntarily voided from male dogs than from female dogs. Urine protein concentration did not correlate with body wt, body surface area or gae. Urine protein concentration did correlate statistically with urine creatinine concentration (r = 0.43) and urine specific gravity (r = 0.49). Mean 24 h urine protein loss in 10 Beagles was higher for the CBB method (mean 70 mg, range 24-197) than for the TCA-PS method (mean 38 mg, range 8-151 mg). The protein/creatinine ratio in the 24 h urine samples was better correlated with 24 h protein loss (r = 0.96) than was protein concentration with 24 h protein loss (r = 0.83). Determination of urine protein/creatinine ratio may be a better predictor of 24 h urine protein loss than measurement of urine protein concentration alone.