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Preparation of human platelets without the use of anticoagulants and study of the effect of Catt on aggregation

Lösche, W.; Hofmann, B.; Hofmann, J.; Spangenberg, P.; Ostermann, G.; Mindner, K.; Till, U.

Folia Haematologica 106(5-6): 870-878

1979

ISSN/ISBN: 0323-4347
PMID: 94881
Document Number: 146817
A method for the preparation of human blood platelets is presented which substitutes the use of anticoagulants by a gelfiltration for the removal of plasma calcium from native blood. In a second step the platelets are separated from the gel filtered blood by a centrifugation on a Ficoll density gradient. The anticoagulant-free platelets reveal an intact morphological feature and a normal aggregation behaviour in response to different aggregation inducers. It was found that in ADP-induced aggregation monophasic aggregation is shifted to a biphasic one by increasing concentrations of extracellular calcium.

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