The location of the C3 and GLO (glyoxalase 1) loci of the IXth linkage group in mice
Rubinstein, P.; Vienne, K.; Hoecker, G.F.
Journal of Immunology 122(6): 2584-2589
1979
ISSN/ISBN: 0022-1767 PMID: 448134 Document Number: 146709
Electrophoretic variants of glyoxalase-1 (GLO) and complement component C3 in the mouse were used to define the genetic location of the corresponding genes. Previous work had demonstrated that these 2 loci are linked to the H-2 complex. The segregation of GLO and H-2 in 880 informative meioses established that the recombination fraction for these 2 genes/complexes is 0.0174 plus or minus 0.005; two intra-H-2 crossovers map GLO on the K-side of the H-2 complex. Similarly, the linkage of C3 to the H-2 complex was confirmed. The recombination fraction was 0.114 plus or minus 0.022. A triple-point cross, involving C3, H-2 complex, and GLO, discloses that the order of these genes is (from the centromere) GLO - H-2 - C3. It is speculated that the centromeric end of the MHC may be the more ancient gene of the system, because it has remained in linkage with GLO.