Stimulation of blood platelets by extracts of subcutaneous tissue
Bender, N.; Kirchmaier, C.; Bartsch, B.; Lindenborn, D.; Breddin, K.
Thrombosis Research 14(2-3): 341-351
1979
ISSN/ISBN: 0049-3848 PMID: 442011 Document Number: 146414
If blood was fixed at blood sampling by feeding it directly into glutaraldehyde, 15-25% of the platelets showed pseudopods and were spherical, while the majority retained their disc-like shape. When the canula was accidentally inserted into the perivascular tissue and then withdrawn to collect venous blood almost all platelets were spherical and showed pseudopods. Similar morphologic changes could be induced with regularity within 1-2 s by admixing s.c. tissue extracts to freshly drawn blood before fixation. The activity which caused these rapid morphologic changes seemed to be independent of ADP or collagen and more active. It did not induce aggregation but may be important for the initiation of primary hemostasis. Similar effects were obtained with lysolecithin and monophosphoinositit/phosphatidylcholine.