Reliability of Mycophenolic Acid Monitoring by an Enzyme Multiplied Immunoassay Technique
Martiny, D.; Macours, P.; Frédéric Cotton; Thiry, P.; Béatrice Gulbis
Clinical Laboratory 56(7-8): 345-353
2010
ISSN/ISBN: 1433-6510 PMID: 20857899 Document Number: 14624
Background: For mycophenolic acid (MPA), substantial inter- and intra-individual variability and drug interactions have been observed and therapeutic drug monitoring is now recommended. In this study, a MPA commercial Enzyme Multiplied Immunoassay Technique (EMIT) was evaluated and compared with the HPLC-UV reference method which is easily practicable in a routine laboratory. Methods: Plasma samples (n=117) were collected from adult renal graft patients treated by mycophenolate in combination with either cyclosporin A (CyA) (n=32) or tacrolimus (n=85). Results: Considering all samples, correlation was excellent (p Conclusions: In the early post-transplant period, or in cases where CyA is used in combination with MPA, the EMIT cannot be recommended. HPLC or LC/MS are here the method of choice.
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