Assessment of pregnancy in sheep by analysis of plasma progesterone using an amplified enzyme immunoassay technique
McPhee, I.M.; Tiberghien, M.P.
Veterinary Record 121(3): 63-65
1987
ISSN/ISBN: 0042-4900 PMID: 3307117 Document Number: 295689
An amplified enzyme immunoassay kit for progesterone analysis was used to diagnose pregnancy in a flock of 130 mule ewes. An accuracy of 100% was obtained after the analysis of progesterone in plasma samples taken 15-16 days after mating. In mule ewes a plasma progesterone level greater than 5.9 nmol/litre was indicative of pregnancy. In the validation of the technique, duplicate ewe plasma samples and progesterone standards were compared with a radioimmunoassay technique; the regression coefficient between the two techniques was r = 0.82.