Metal--glutathione interaction in aqueous solution. Nickel (II) , cobalt (II) and copper (II) complexes with oxidized glutathione
Formicka-Kozłowska, G.; Kozłowski, H.; Jezowska-Trzebiatowska, B.
Acta Biochimica Polonica 26(3): 239-248
1979
ISSN/ISBN: 0001-527X PMID: 227199 Document Number: 145143
The interaction of copper(II), nickel(II) and cobalt(II) ions with oxidized glutathione in aqueous solutions have been examined by spectroscopic methods. Cu(II) is the only ion which interacts with disulphide bridge and forms dimeric species containing the Cu(II)-S-S-Cu(II) unit. Ni(II) and Co(II) bind mainly with the terminal NH2 and COO- groups of glutamic acid, and the complexes formed are of nearly octahedral symmetry. At high pH, in the Co(II)-GSSG solution Co(II) is oxidized to Co(III) with the concomitant reduction of GSSG to GSH. Considerable differences were observed between the oxidized and reduced form of glutathione in the coordination ability towards metal ions.