Glutathione metabolism in canine hereditary stomatocytosis with mild erythrocyte glutathione deficiency
Smith, J.E.; Moore, K.; Boyington, D.; Potter, K.A.
Journal of Laboratory and Clinical Medicine 101(4): 611-616
1983
ISSN/ISBN: 0022-2143 PMID: 6833832 Document Number: 204657
Mild glutathione deficiency can accompany hereditary stomatocytosis in man and dogs. Several facets of glutathione metabolism were studied in dogs to better define hereditary stomatocytosis. In vivo glutathione flux was measured with .alpha.-ketoglutarate-.alpha.(2-14C] and glycine-(2-3H) in a double-labeled experiment. Glycine-labeled glutathione turnover was faster than that of .alpha.-ketoglutarate-labeled glutathione, and the glutathione flux was greater in HSt cells than in normal cells. The glutathine-reducing enzymes, glutathione reduction rate, glutathione precursors (except cysteine), oxidized glutathione percentage and erythrocyte H2O were increased significantly in HSt cells. Glutathione synthetic enzymes, oxidized glutathione transport system and glutathione-S-transferase did not differ significantly. The mild glutathione deficiency in canine hereditary stomatocytosis apparently is a reflection of increased catabolism.