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Anti-HBs antibody. a new immunoenzymatic detection. Preliminary results

Cohen, J.; Joyce, L.; Brassart, G.; Ruiz, J.C.

Annales de Biologie Clinique 37(5): 291-293

1979

ISSN/ISBN: 0003-3898
PMID: 398165
Document Number: 145091
The detection of anti-HBs antibody has been carried out using a new technique where a system for the detection of HBs antigen in the solid phase is adopted to demonstrate the presence of anti HBs antibody by inhibition of the immunoenzymatic reaction. The sensitivity of the method has been compared: with that of passive haemagglutination and with that of radioimmunoassay in liquid phase, for human serum obtained from 10 chronic antibody carriers and also serum from 1 rabbit, with that of solid phase radioimmunoassay for 91 positives serums. The method appears: in the range of sensitivity of passive haemagglutination and radioimmunoassay in the liquid phase, less sensitive than that of solid phase radioimmunoassay, far superior to that of electrosyneresis.

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