Anti-HLA antibody detection and rejection in kidney transplantation: impact of the new technologies
Georgescu, D.; Ferrari-Lacraz, S.; Villard, J.
Revue Medicale Suisse 3(108): 1064-1069
2007
ISSN/ISBN: 1660-9379 PMID: 17552259 Document Number: 608939
In kidney transplantation, hyperacute rejection is mediated by anti-HLA antibody which are also responsible for antibody-mediated acute rejection. In addition anti-HLA antibody are also implicated in the physiopathological mechanism of chronic rejection. The gold standard methodology to detect anti-HLA antibody is based on the complement-dependant-cytotoxicity. This technic is neither specific nor sensitive. New powerful technologies, which are specific and very sensitive, have been developed like Elisa and flow cytometer with fluorescent micro-beads to detect anti-HLA antibody. In this article, we review the importance of anti-HLA antibody in humoral rejection. We also discussed the clinical relevance of the detection of anti-HLA antibody by these new approaches.