Production of monoclonal anti-thymine-dimer antibody and its use for detection of STR alleles
Fukuda, M.; Tamaki, Y.; Kishida, T.; Wang, W.
Nihon Hoigaku Zasshi 50(5): 343-348
1996
ISSN/ISBN: 0047-1887 PMID: 8952334 Document Number: 457546
To simplify the manual detection of STRs, we developed an immunoblotting method using an anti-thymine-dimer antibody. Upon ultraviolet irradiation, adjacent thymine residues in a DNA strand readily form thymine dimers which are detectable by anti-thymine-dimer antibody. We simply irradiated the supernatant of a boiled suspension of P3U1 myeloma cells with ultraviolet rays, and immunized BALB/c mice with it. By fusing their popliteal lymph node cells with the P3U1 myeloma cell line, we produced a monoclonal anti-thymine-dimer antibody. Using this antibody, we were able to detect STR alleles containing AAAG, AAGG, or AATG repeats by immunoblotting. Our method is well suited for use in parentage testing.