Effects of ischaemia on enzyme-activities in the soleus muscle of the rat
Bass, A.; Gutmann, E.; Hanzlíková, V.; Teisinger, J.
Pflugers Archiv European Journal of Physiology 379(2): 203-208
1979
ISSN/ISBN: 0031-6768 PMID: 571116 Document Number: 143522
Complete ischemia was produced in the rat soleus muscle by section of the abdominal aorta and terminal devascularization, leaving nerve and tendon intact. During the 2 wk ischemia the activities of energy-supplying enzymes and of the hydrolytic enzyme, acid phosphatase, were studied. Activities of glycolytic enzymes, oxidative enzymes, hexokinase and acid phosphatase are affected in a different manner. Activities of the glycolytic enzymes, lactate dehydrogenase, triosephosphate dehydrogenase and glycerolphosphate dehydrogenase, are lowest on the 1st day and increase thereafter. Lactate dehydrogenase and triosephosphate dehydrogenase reach the control values on the 4th and 14th day, respectively, while glycerolphosphate dehydrogenase reaches about 50% of the control value on the 14th day. The maximum decrease in activity of the oxidative enzymes, citrate synthase, .beta.-hydroxyacyl-CoA-dehydrogenase and malate dehydrogenase occurs later (4th day); thereafter their activity returns slowly to control values, but does not reach them even on the 14th day. Hexokinase activity is slightly decreased on the 1st day; then it increased to twice the control value by 7th day. On the 1st day the activity of the aerobic metabolism enzymes prevail, and on the 4th day those of anaerobic carbohydrate (glucose) metabolism; the recovery of enzyme activity of aerobic oxidation occurs later. Acid phosphatase activity increased from the 2nd day onwards, reaching up to 3 times the control value on the 4th day and still twice that value on the 14th day. Histochemical changes of alkaline phosphatase activity showed destruction of capillary endothelial cells during the 1st few days after operation and their later proliferation from the periphery, correlating with the loss and recovery of oxidative enzyme activity.