DC polarography of cephradine and its application to capsules
Nuñez-Vergara, L.J.; Squella, J.A.; Gonzalez, E.M.
Journal - Association of Official Analytical Chemists 62(3): 556-559
1979
ISSN/ISBN: 0004-5756 PMID: 479077 Document Number: 142485
A polarographic method has been developed for the quantitative analysis of cephradine and its dosage forms. Direct determinations on capsules are carried out; excipients and coloring matter do not interfere in the determination. The electroactive product is formed by acidic hydrolysis with 5.0N HCl and heating at 80 degrees C for 60 min. Two polarographic waves are obtained: I = -0.46 V and II = -0.78 v vs. SCE. Both reduction waves are diffusion controlled. Wave I is preferred for analytical purposes. The precise chemical identity of the electroactive product has not been determined, but UV spectral data and the TLC Rf value are reported. A linear relation is established for levels of cephradine between 10(-2) and 10(-5)M in 5.0N HCl.