The isoelectric point of thrombin and its behaviour compared to prothrombin at some solid surfaces
Berg, W.; Hillvärn, B.; Arwin, H.; Stenberg, M.; Lundström, I.
Thrombosis and Haemostasis 42(3): 972-982
1979
ISSN/ISBN: 0340-6245 PMID: 41337 Document Number: 141768
The isoelectric point of thrombin was high and thrombin was a cation at the pH of blood. Prothrombin had a low isoelectric point, being more anionic at the pH of blood. Thrombin adsorbed readily to surfaces, especially negatively charged surfaces, like behenic acid surfaces at pH 8.2. Thrombin adsorbed onto behenic acid was active in the sense that it coagulated fibrinogen. The significance of the electric charge of the thrombin molecule in the mechanism of atherosclerosis was discussed.
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