Carboxylation enzymes of coryneform bacteria
Baryshnikova, L.M.; Loginova, N.V.
Mikrobiologiia 48(6): 965-968
1979
ISSN/ISBN: 0026-3656 PMID: 119147 Document Number: 141145
The enzymes of CO2 heterotrophic fixation were studied in 6 strains of coryneform bacteria belonging to the genera Arthrobacter, Brevibacterium, Corynebacterium and Norcardia. All of the strains contained PEP (phosphoenolpyruvate) carboxylase (EC 4.1.1.31), NADP or NAD dependent malic enzymes (EC 1.1.1.38-40). Pyruvate carboxylase (EC 6.4.1.1) was found only in 3 strains of coryneforms: B. ammoniagenes, C. aquaticum and N. erythropolis. PEP carboxykinase (EC 4.1.1.32) was detected in B. ammoniagenes and N. erythropolis. PEP carboxyphosphotransferase (EC 4.1.1.38) was found only in B. ammoniagenes. These data suggest that carboxylation of C3-acids is 1 of the essential pathways in some coryneforms supplying the citric acid cycle with the products of glycolysis. The composition and the level of carboxylation enzymes reflect the ecological characteristics of the organisms rather than their taxonomical relations.