Cephaloridine and the rat kidney lysosomes stability in vitro
Ngaha, E.O.
West African Journal of Pharmacology and Drug Research 5(1): 25-35
1979
ISSN/ISBN: 0303-691X PMID: 555127 Document Number: 140497
Two methods employing lysosomal enzyme release and light scattering measurements showed that cephaloridine stabilizes rat kidney lysosomes in vitro against .beta.-progesterone and lysolecithin, the 2 well-known agents to labilize lysosomal membranes. Treatment of the isolated lysosomes suspended in 0.25 M-sucrose-EDTA (0.34 mM) pH 7.4 with cephaloridine (100 .mu.M) at 37.degree. C for 10 min and subsequent incubation of the mixture with progesterone (0.25 mM) at the same temperature for varying periods of time up to 50 min caused no significant changes from the normal values in the release of acid phosphatase activity obtained from the supernatant fraction after centrifugation of the incubated mixture at 33,000 g for 5 min at 4.degree. C. When lysosomes were mixed with cephaloridine before treatment with progesterone or lysolecithin and subjected to light scattering measurements, lysosomal swelling was considerably decreased; cephaloridine could exert a stabilizing effect on rat kidney lysosomal membrane and thereby limit the release of lysosomal acid hydrolases which are involved in acute inflammatory processes.