Top Abstract Purchase Related
Home
>
Node
>
Subnode

Studies on a pituitary factor which stimulates DNA synthesis in isolated thymocytes: Part IV--Effect of PF on the cell size of thymocytes

Saxena, Q.B.; Saxena, R.K.

Indian Journal of Biochemistry and Biophysics 16(4): 204-208

1979

ISSN/ISBN: 0301-1208
PMID: 43834
Document Number: 140436
The effect of the pituitary factor (PF) isolated from sheep pituitary glands on the cell size distribution pattern of thymocytes was studied using a Coulter Counter. It induces an enlargement of cell volume in isolated thymocytes . A procedure for the quantification of this effect of PF is described. The effect of PF is dose-dependent and is observed within half an hour of incubation. Maximum effect was obtained on thymocytes though a significant effect was also observed on cell derived from spleen and bone marrow. PF did not induce immunocompetence in thymocytes as measured by their graft vs. host reactivity in chick embryo system. The effect of PF on cell size could not be due to maturation of these cells. The swelling of cells may be related to the triggering of these cells to divide by PF.

Document emailed within 1 workday
Secure & encrypted payments

Saxena, Q.B.; Saxena, R.K. 1978: Studies on a pituitary factor which stimulates DNA synthesis in isolated thymocytes: Part III--Effect on cultured thymocytes and embryonic thymus lobes maintained in organ culture Indian Journal of Biochemistry and Biophysics 15(6): 439-442
Saxena, R.K. 1978: Studies on a primary factor which stimulates DNA synthesis in isolated thymocytes: Part II--Bioassay, partial purification and characterization Indian Journal of Biochemistry and Biophysics 15(6): 433-438
Kienker, L.J.; Kuziel, W.A.; Garni-Wagner, B.A.; Kumar, V.; Tucker, P.W. 1991: T cell receptor gamma and delta gene rearrangements in scid thymocytes. Similarity to those in normal thymocytes Journal of Immunology 147(12): 4351-4359
Håkansson, L. 1975: Effect of cytostatic drugs on nucleic acid synthesis in mouse thymocytes and chick embryo cells Arzneimittel-Forschung 25(3): 378-382
Golikov, P.P.; Bobkova, A.S.; Kamernitskiĭ, A.V.; Reshetova, I.G. 1982: Effect of transformed steroid compounds on mRNA synthesis and glucocorticoid-receptor interaction in thymocytes of adrenalectomized rats Farmakologiia i Toksikologiia 45(2): 89-93
Willcox, H.N.; Newsom-Davis, J.; Calder, L.R. 1984: Cell types required for anti-acetylcholine receptor antibody synthesis by cultured thymocytes and blood lymphocytes in myasthenia gravis Clinical and Experimental Immunology 58(1): 97-106
Godlewski, A.; Chilarski, A.; Brzeziński, P.M.; Dobek-Smiech, J.; Bułhak-Guz, H. 1996: Unilateral testicular torsion in Lewis rats. Part II: the effect on thymocytes mitotic activities in vivo and some morphometric features of thyroid gland Folia Morphologica 55(4): 264-266
Blomgren, H. 1973: Synergism between thymocytes and lymph node cells in the graft-verses-host response effect of cortisone treatment of the thymus cell donor Journal of Immunology 110(1): 144-147
Zherbin, E.A.; Khanson, K.P.; Vashchenko, V.I.; Reshchikov, A.M.; Sungurov, A.I. 1981: Various biochemical and biophysical properties of a subpopulation of rat thymocytes isolated in a Ficoll gradient Biulleten' Eksperimental'noi Biologii i Meditsiny 91(10): 434-436
Mizushima, Y.; Saitoh, M.; Ogata, M.; Kosaka, H.; Tatsumi, Y.; Kiyotaki, C.; Hamaoka, T.; Fujiwara, H. 1989: Thymic stroma-derived T cell growth factor (TSTGF). IV. Capacity of TSTGF to promote the growth of L3T4- Lyt-2- thymocytes by synergy with phorbol myristate acetate or various IL Journal of Immunology 142(4): 1195-1202
Suda, T.; Zlotnik, A. 1991: IL-7 maintains the T cell precursor potential of CD3-CD4-CD8- thymocytes Journal of Immunology 146(9): 3068-3073
D'Adamio, L.; Clayton, L.K.; Awad, K.M.; Reinherz, E.L. 1992: Negative selection of thymocytes. A novel polymerase chain reaction-based molecular analysis detects requirements for macromolecular synthesis Journal of Immunology 149(11): 3550-3553
Pereverzev, B.L.; Ovsepian, E.R.; Konradov, A.A.; Aleshchenko, A.A. 1976: Cytophotometric analysis of phases in the cell cycle of mouse thymocytes Tsitologiia 18(9): 1130-1133
Hanson, D.F.; Murphy, P.A.; Silicano, R.; Shin, H.S. 1983: The effect of temperature on the activation of thymocytes by interleukins i and Ii Journal of Immunology 130(1): 216-221
Potapova, G.I.; Khramtsova, S.N.; Dmitrieva, L.V.; Shapot, V.S. 1986: Changes in the lymphoid cells of DNA and purine nucleotide synthesis and sensitivity to glucocorticoids associated with impairment of differentiation and immune function during tumor growth in mice. Thymocytes Biokhimiia 51(3): 516-526
Timoshenko, A.V.; Zorin, V.P.; Cherenkevich, S.N. 1988: Effect of concanavalin a on the level of sulfhydryl groups in thymocytes Ukrainskii Biokhimicheskii Zhurnal 60(1): 41-46
Nakashima, I.; Zhang, Y.H.; Rahman, S.M.; Yoshida, T.; Isobe, K.; Ding, L.N.; Iwamoto, T.; Hamaguchi, M.; Ikezawa, H.; Taguchi, R. 1991: Evidence of synergy between Thy-1 and CD3/TCR complex in signal delivery to murine thymocytes for cell death Journal of Immunology 147(4): 1153-1162
Verschuren, M.C.; van Bergen, C.J.; van Gastel-Mol, E.J.; Bogers, A.J.; van Dongen, J.J. 1996: A DNA binding protein in human thymocytes recognizes the T cell receptor-delta-deleting element psi J alpha Journal of Immunology 156(10): 3806-3814
Knopp, J.; Straková, Z. 1985: The effect of forskolin and triiodothyronine on the production of cyclic adenosine monophosphate in the thymocytes of rats Bratislavske Lekarske Listy 83(3): 323-329
Dmitrenko, N.P.; Goroshnikova, G.V. 1987: Metabolism of extracellular adenine nucleotides and adenosine uptake by rat thymocytes; the effect of concanavalin A Ukrainskii Biokhimicheskii Zhurnal 59(2): 61-65