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Variability of the C-segment sizes of chromosomes 1, 9, 16 and y in the human chromosome set

Podugol'nikova, P.A.; Sycheva, Z.L.; Prokof'eva-Bel'govskaia, A.A.

Tsitologiia 21(6): 667-670

1979

ISSN/ISBN: 0041-3771
PMID: 462543
Document Number: 139289
The investigation of chromosome polymorphism by quantitative methods is a rather hard task. The manual method for measuring C-segments of chromosomes 1, 9, 16 and Y in man is suggested, which is not difficult, being reasonably precise for the population research. Metaphases of the average level of chromosome condensation were taken for analysis. Only the C-segments were measured without measuring chromosomes. The negative chromosome image was 4000-fold magnified, compared to the chromosome natural size, and the boundaries of C-segments of each chromosome were five-fold dotted on a sheet of paper specially printed for this purpose. C-segments were measured by magnifying glass with 0.025 mcm scale unit. For every individuum, C-segments were measured in 5-7 cells only. The data are presented on the estimation of measurement errors and on individual (intercellular) and population (interindividual) variations of C-segments of chromosomes.

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