Radioimmunoassay [RIA] of cholecystokinin-pancreozymin [33-CCK
Schlegel, W.; Grube, D.
Zeitschrift für Gastroenterologie 16(5): 304-310
1978
ISSN/ISBN: 0044-2771 PMID: 654386 Document Number: 139023
A further specific, reproducible radioimmunoassay of cholecystokinin-pancreozymin was developed. 125J-labelled 39-CCK was used as tracer. The limit of detection for CCK in serum was 40 pg/ml. Basal levels for CCK have been found to be 222 pg/ml, being increased after food ingestin. No crossreactivity with gastronintestinal hormones could be observed. Somatostatin was inhibiting the stimulated CCK-release. Histochemical and radioimmunological determinations localized CCK-containing cells in duodenal tissue of humans. Physiological and pathological processes in which CCK is involved have to be studied to evaluate its role in the gastrointestinal tract.