Heterogeneity of leghemoglobin from yellow lupine nodules
Kudriavtseva, N.N.; Borodenko, L.I.; Krasnobaeva, N.N.; Zhizhevskaia, G.I.
Biokhimiia 43(2): 321-326
1978
ISSN/ISBN: 0320-9725 PMID: 647081 Document Number: 138485
A possibility is demonstrated to separate summary lupine leghemoglobins (which are salted out within 55--90% of ammonium sulphate saturation) into Lb I and Lb II components by means of ionic exchange chromatography on DEAE-cellulose. Lb I is eluted at lower ionic strength buffer than LbII, and differs from the latter in the form and the size of crystals. Both components have the same electrophoretic mobility and contain N-terminal glycine. LbII and LbI precipitate under gradual salting out within 55--75% and 78--90% of saturation respectively.