Effect of sodium n-dipropylacetate (sodium valproate) on GABA level of neuronal and glial cells in culture
Gensburger, C.; Simler, S.; Ciesielski, L.; Mandel, P.
Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales 172(4): 701-706
1978
ISSN/ISBN: 0037-9026 PMID: 218700 Document Number: 137570
The effect of sodium n dipropylacetate (nDPA), a competitive GABA-T inhibitor with respect to GABA, has been investigated on glial and neuronal cellular GABA level. After 1 to 4 days incubation with nDPA in the culture medium, a decrease of GABA level in M5 neuroblastoma clonal cell lines and no modification of GABA level in C6 astrocytoma cells has been observed. The combined addition of nDPA 4 micrometer with dibutyryl cyclic AMP (1 mM) to the culture medium induces the same decrease in GABA level in C6 astrocytoma cells as the addition of DB-c-AMP alone. After shorter incubation time with nDPA (5-150 min), we observed a decreased GABA level in C6 astrocytoma glial cells.