Common membrane neoantigens on bovine papilloma virus-induced fibroma cells from cattle and horses
Barthold, S.W.; Olson, C.
American Journal of Veterinary Research 39(10): 1643-1645
1978
ISSN/ISBN: 0002-9645 PMID: 213996 Document Number: 137542
Cultured cells from bovine papilloma virus (BPV)-induced fibroblastic tumours and the normal dermis of cattle, horses, and hamsters were examined for cell membrane or internal neoantigens, using the indirect immunofluorescence technique. Serum samples from cattle and horses bearing BPV-induced fibromas cross reacted with cell membranes of tumour, but not with normal dermal cells of both species. The reaction could be blocked with homologous, but not heterologous, serum of these two species. Immunofluorescence was not detected with serum from hamsters bearing BPV-induced sarcomas if incubated with bovine, equine, or hamster cells. Internal neoantigens were not found in any of the acetone-fixed tumour cells, using serum from the three species. Both tumour and normal cells were all found free of BPV antigen, using direct immunofluorescence.