Dynamics of the restoration of a population of peritoneal mast cells following their destruction
Kuznetsov, S.A.; Chernyshova, E.V.
Arkhiv Anatomii Gistologii i Embriologii 74(2): 89-93
1978
ISSN/ISBN: 0004-1947 PMID: 646643 Document Number: 135506
Removal of the mast cells from the mouse abdominal cavity by repeated washings as well as their osmotic destruction with distilled water are suitable methods to study regeneration of mast cells in postnatal ontogenesis. Simultaneous application of their differential staining with alcian blue-safranin makes it possible to reveal successive stages of the newly formed mast cells maturation and determine duration of every stage. The time of complete renovation of the peritoneal mast cells has been stated to be, according to the mode of the stimulation, 60--80 days. Total duration of the first two stages of maturation is 1--2 days. Mast cell differentiation is fully completed by the 11th day.