A sensitive gas chromatograph assay for thiopentone in plasma
Van Hamme, M.J.; Ghoneim, M.M.
British Journal of Anaesthesia 50(2): 143-145
1978
ISSN/ISBN: 0007-0912 PMID: 626695 Document Number: 135219
A sensitive flame ionization gas chromatograph assay method was developed for thiopentone in human plasma. The extraction procedure minimizes desulfuration of the drug while removing most endogenous substances and is limited by a maximum of 50 ng/ml-1 in 2-ml plasma samples. There is linearity throughout the 30.0-5.0 .mu.g ml-1 and 5.0-0.1 .mu.g ml-1 ranges. Extraction of 6 replicate 2.5 .mu.g ml-1 samples resulted in a coefficient of variation of 3.0%. Similar treatment of 6 0.20-.mu.g ml-1 samples afforded a coefficient of variation of 4.6%.