A sensitive assay for Staphylococcus aureus nucleases
Kohli, J.K.; Vakil, B.V.; Patil, M.S.; Pandey, V.N.; Pradhan, D.S.
Indian Journal of Biochemistry and Biophysics 26(5): 296-300
1989
ISSN/ISBN: 0301-1208 PMID: 2628267 Document Number: 346263
A sensitive assay for staphylococcal nuclease involving incubation of the enzyme sample with heat-denatured [3H]thymidine labelled DNA from Escherichia coli, precipitation with trichloroacetic acid and measurement of the radioactivity of acid-soluble nucleotides released has been developed. The assay is sensitive enough to be used for comparing the levels of nucleses elaborated by different strains of S. aureus as well as for determining the extent of contaminaiton of S. aureusin food and water samples even at levels at which the conventional spectrophotometric and toluidine blue-DNA methods are totally inadequate.