The development and testing of reagents kit for detection and qualitative evaluation of DNA of methicillin sensitive and methicillin resistant Staphylococcus aureus and also methicillin resistant coagulase negative Staphylococcus spp. applying technique of polymerase chain reaction in "real time" mode
Skachkova, T.S.; Shipulina, O.I.; Domonova, É A.; Subbotovskaia, A.I.; Kozyreva, V.S.; Il'ina, V.N.; Shipulin, G.A.
Klinicheskaia Laboratornaia Diagnostika 6: 42-45
2013
ISSN/ISBN: 0869-2084 PMID: 24340947 Document Number: 666056
The reagents kit is developed to identify and quantitatively detect DNA of methicillin sensitive and methicillin resistant Staphylococcus aureus, methicillin resistant coagulase negative Staphylococcus spp. in biological material using technique of polymerase chain reaction with hybridizational fluorescent detection and having higher analytical and diagnostic characteristics. The application of the given reagents kit makes it possible to optimize the epidemiologic monitoring of propagation of methicillin resistant strains of Staphylococcus spp. Significantly decreasing duration and laboriousness of study.