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Genetic properties of the Salmonella enteritidis R404 plasmid aggregate. II. Separation of plasmids by transformation

Cebrat, S.; Kurowska, E.; Rogoliński, J.

Acta Microbiologica Polonica 27(3): 203-211

1978

ISSN/ISBN: 0137-1320
PMID: 81594
Document Number: 132667
Transformational separation of plasmids from R404 plasmid aggregate found in Salmonella enteritidis strain was performed. Three classes of transformants differing in their resistance patterns were isolated. Genetic properties of the transformants suggest that their resistance is determined by single plasmids. Plasmid pCK3 (Tra-ApCbCrSuSm) and pCK4 (Tra-ApCbCrCm) are nonconjugative while plasmid pCG1 (TraApCbCrSuSmTcKmNm) is conjugative. Separation of all plasmids of R404 plasmid aggregate allowed to determine their genetic properties and the manner of conjugational transfer of R404 plasmid aggregate R-determinants.

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