Repercussions on hepatic lipogenesis in the rat during realimentation with diets of different protein content
Carrozza, G.; Livrea, G.; Russo, C.R.
Bollettino della Societa Italiana di Biologia Sperimentale 54(24): 2488-2492
1978
ISSN/ISBN: 0037-8771 PMID: 756266 Document Number: 131598
To study the relationship between protein diets and liver lipogenesis, male Wistar rats were 1st put on a normal protein diet, then on diets with varying amounts of protein. The animals were sacrificed and spectrophotometric assays of various enzymes in liver samples were done. The changes in 6 enzyme parameters in rat liver determined during the fasting period before the change in diet, and during subsequent normoprotein, 0 protein and hyperprotein diets are listed. A normal protein diet increased all liver enzymes tested, while the preliminary fasting period depressed them, as did a non-protein diet. A non-protein or a hyperprotein diet inhibited lipogenesis and lipogenic enzymes, with the exception of acetyl-CoA carboxylase. This enzyme and fatty acid synthetase are the limiting enzymes of lipogenesis. The concentration of of acyl-CoA, noted inhibitor of lipogenesis, diminished as the protein supply was reduced.